Fluorescence in situ hybridization (FISH) analysis was performed on a
formalin-fixed Block 1A (Breast Cancer) using the DNA probe for the HER-2/NEU
gene (Vysis, Inc., lot # 417686). An adequate number of invasive tumor cells
were present and evaluated by two independent observers. The ratio of
HER-2/NEU signals (ERBB2) to chromosome 17 centromere signals (D17Z1) was
determined to be 1.26. A ratio of greater than 2.2 is considered to be
amplified; therefore, this specimen is not amplified. Although amplification
was not seen, many of the cells exhibited 3 or more signals for both the
HER-2/NEU gene and for the chromosome 17 centromere. This may be indicative
of either polyploidy or aneuploidy for chromosome 17. The average number of
HER-2/NEU signals per cell was 5.83. The average number of signals for the
chromosome 17 centromere was 4.63.
formalin-fixed Block 1A (Breast Cancer) using the DNA probe for the HER-2/NEU
gene (Vysis, Inc., lot # 417686). An adequate number of invasive tumor cells
were present and evaluated by two independent observers. The ratio of
HER-2/NEU signals (ERBB2) to chromosome 17 centromere signals (D17Z1) was
determined to be 1.26. A ratio of greater than 2.2 is considered to be
amplified; therefore, this specimen is not amplified. Although amplification
was not seen, many of the cells exhibited 3 or more signals for both the
HER-2/NEU gene and for the chromosome 17 centromere. This may be indicative
of either polyploidy or aneuploidy for chromosome 17. The average number of
HER-2/NEU signals per cell was 5.83. The average number of signals for the
chromosome 17 centromere was 4.63.